ELECTRON MICROSCOPY IN MICROBIOLOGY

This practical guide provides detailed descriptions of electron micros copic techniques relevant to all fields of microbiological research. H olzenberg describes all standard techniques required for the structura l characterization of unicellular organisms (suspended or fixed in mic robial biofilms) and subcellular components, including enzymes, nuclei c acids, and inclusion bodies. Particular attention is given to such t echniques as epitope mapping and localization of proteins by antibodie s and other marker systems that provide information on the structure a nd compartmentation of biological macromolecules within the cell.